Round 23
|
| Thursday, 4 September 2008 |
| FC Dallas | 0 | Colorado Rapids | 1 |
| | | 60 Petke | |
| Saturday, 6 September 2008 |
| San Jose | 2 | D.C. United | 1 |
| 18 Alvarez | | 76 Quaranta | |
| 62 Huckerby | | | |
| L.A. Galaxy | 2 | Real Salt Lake | 2 |
| 9 Jordan | | 21 Movsisyan | |
| 69 Buddle | | 52 Johnson | |
| Chicago Fire | 1 | New York | 0 |
| 36 King | | | |
| Toronto FC | 1 | Chivas USA | 3 |
| 30 Rosenlund | | 41 Palladini | |
| | | 58 Bornstein | |
| | | 90 Razov | |
| Columbus Crew | 4 | N.E. Revolution | 0 |
| 40 Barros Schelotto | | | |
| 45 Iro | | | |
| 67 Garey | | | |
| 90 Garey | | | |
| Sunday, 7 September 2008 |
| Houston Dynamo | 3 | K.C. Wizards | 1 |
| 28 Davis | | 87 Arnaud | |
| 54 Jaqua | | | |
| 67 Jaqua | | | |
Round 24
|
| Thursday, 11 September 2008 |
| N.E. Revolution | 4 | Chivas USA | 0 |
| 53 Twellman | | | |
| 83 Larentowicz | | | |
| 90 Ralston | | | |
| 90 Smith | | | |
| Saturday, 13 September 2008 |
| New York | 2 | Real Salt Lake | 1 |
| 49 Ángel | | 38 Movsisyan | |
| 64 Van Den Bergh | | | |
| Toronto FC | 1 | Columbus Crew | 1 |
| 6 Robinson | | 40 Noonan | |
| San Jose | 1 | Houston Dynamo | 1 |
| 50 O'Brien | | 71 Ching | |
| K.C. Wizards | 2 | L.A. Galaxy | 0 |
| 67 Wolff | | | |
| 69 Arnaud | | | |
| D.C. United | 2 | FC Dallas | 2 |
| 26 Moreno | | 9 Cooper | |
| 69 Quaranta | | 79 André Rocha | |
| Sunday, 14 September 2008 |
| Colorado Rapids | 2 | Chicago Fire | 0 |
| 30(og) Busch | | | |
| 37 Cummings | | | |
Round 25
|
| Thursday, 18 September 2008 |
| Columbus Crew | 3 | New York | 1 |
| 41 Hejduk | | 21 Ángel | |
| 46 Rogers | | | |
| 85 Gaven | | | |
| Saturday, 20 September 2008 |
| K.C. Wizards | 2 | Toronto FC | 0 |
| 63(p) Jewsbury | | | |
| 79 Souter | | | |
| L.A. Galaxy | 5 | D.C. United | 2 |
| 23 Donovan | | 2 McTavish | |
| 35 Donovan | | 48 Khumalo | |
| 38 Gordon | | | |
| 81 Vagenas | | | |
| 90 Donovan | | | |
| Real Salt Lake | 0 | Chivas USA | 1 |
| | | 72 Eskandarian | |
| Colorado Rapids | 1 | N.E. Revolution | 1 |
| 42 Clark | | 50 Mansally | |
| Sunday, 21 September 2008 |
| Chicago Fire | 1 | FC Dallas | 4 |
| 90 McBride | | 2 Cunningham | |
| | | 43 Pitchkolan | |
| | | 45 Guarda | |
| | | 61 Cunningham | |
Round 26
|
| Thursday, 25 September 2008 |
| Chicago Fire | 3 | L.A. Galaxy | 1 |
| 13 Rolfe | | 35 Lewis | |
| 57 Blanco | | | |
| 59 Rolfe | | | |
| Saturday, 27 September 2008 |
| Toronto FC | 1 | Houston Dynamo | 1 |
| 73 Wynne | | 23(og) Freeman | |
| N.E. Revolution | 0 | Columbus Crew | 1 |
| | | 35 Marshall | |
| Chivas USA | 2 | K.C. Wizards | 1 |
| 26 Eskandarian | | 70 Wolff | |
| 46 Victorine | | | |
| New York | 4 | Colorado Rapids | 5 |
| 5 Magee | | 3 Cummings | |
| 34(p) Ángel | | 11 Casey | |
| 40 Richards | | 43 Petke | |
| 74 Mbuta | | 45(p) Casey | |
| | | 90 Casey | |
| San Jose | 2 | Real Salt Lake | 3 |
| 23 Huckerby | | 3 Movsisyan | |
| 47 Huckerby | | 49 Movsisyan | |
| | | 59 Morales | |
| Sunday, 28 September 2008 |
| FC Dallas | 3 | D.C. United | 0 |
| 9 André Rocha | | | |
| 56 Cunningham | | | |
| 90 Cooper | | | |
Major League 2008